Review



lb nutrient agar medium containing ampicillin  (Beijing Solarbio Science)


Bioz Verified Symbol Beijing Solarbio Science is a verified supplier
Bioz Manufacturer Symbol Beijing Solarbio Science manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Beijing Solarbio Science lb nutrient agar medium containing ampicillin
    Mics of antibiotics and compounds.
    Lb Nutrient Agar Medium Containing Ampicillin, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lb+nutrient+agar+medium+containing+ampicillin/lb+nutrient+agar+medium+containing+ampicillin/pmc10464524-64-15-16
    Average 90 stars, based on 1 article reviews
    lb nutrient agar medium containing ampicillin - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China"

    Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2023.1158659

    Mics of antibiotics and compounds.
    Figure Legend Snippet: Mics of antibiotics and compounds.

    Techniques Used:

    Relative changes in resistance gene mRNA levels in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 under the influence of antibiotics. (A,B) Relative changes in bla CTX–M–14 and bla CTX–M–64 mRNA levels in strain Ec15103 under different concentrations of cefotaxime (CTX); bla CTX–M–14 is present on the Ec15103 plasmid pEc15103B and bla CTX–M–64 on the Ec15103 chromosome. (C) Relative changes in bla CTX–M–14 mRNA levels in strain Ec15103-Ec600 at different concentrations of CTX. bla CTX–M–14 is located on the Ec15103-Ec600 conjugate plasmid pEc15103B. (D) Relative changes in bla TEM–1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of ampicillin (AMP). bla TEM–1 is located on the Ec15103-EPI300-1 transconjugant plasmid pEc15103A. For at least two independent experiments, each with at least three biological replicates, the data shown represent the mean standard deviation using one-way ANOVA.
    Figure Legend Snippet: Relative changes in resistance gene mRNA levels in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 under the influence of antibiotics. (A,B) Relative changes in bla CTX–M–14 and bla CTX–M–64 mRNA levels in strain Ec15103 under different concentrations of cefotaxime (CTX); bla CTX–M–14 is present on the Ec15103 plasmid pEc15103B and bla CTX–M–64 on the Ec15103 chromosome. (C) Relative changes in bla CTX–M–14 mRNA levels in strain Ec15103-Ec600 at different concentrations of CTX. bla CTX–M–14 is located on the Ec15103-Ec600 conjugate plasmid pEc15103B. (D) Relative changes in bla TEM–1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of ampicillin (AMP). bla TEM–1 is located on the Ec15103-EPI300-1 transconjugant plasmid pEc15103A. For at least two independent experiments, each with at least three biological replicates, the data shown represent the mean standard deviation using one-way ANOVA.

    Techniques Used: Plasmid Preparation, Standard Deviation

    Related Articles

    Plasmid Preparation:

    Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China
    Article Snippet: 200 rpm followed by growth to OD 600 ≈1.0, and then 500 μL of donor and recipient bacteria were mixed. The mixture was spotted onto a 1-cm 2 filter membrane placed on LB nutrient agar (Solarbio Science & Technology, Beijing, China). The plates were incubated at 37°C for 12∼18 h, then the cells were washed from the filter membrane to 1 mL of LB broth. After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China). The colonies after overnight culture at 37°C were transconjugants (Ec15103-Ec600), as verified by PCR.

    Standard Deviation:

    Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China
    Article Snippet: 200 rpm followed by growth to OD 600 ≈1.0, and then 500 μL of donor and recipient bacteria were mixed. The mixture was spotted onto a 1-cm 2 filter membrane placed on LB nutrient agar (Solarbio Science & Technology, Beijing, China). The plates were incubated at 37°C for 12∼18 h, then the cells were washed from the filter membrane to 1 mL of LB broth. After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China). The colonies after overnight culture at 37°C were transconjugants (Ec15103-Ec600), as verified by PCR.

    Transmission Electron Microscopy:

    Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China
    Article Snippet: 200 rpm followed by growth to OD 600 ≈1.0, and then 500 μL of donor and recipient bacteria were mixed. The mixture was spotted onto a 1-cm 2 filter membrane placed on LB nutrient agar (Solarbio Science & Technology, Beijing, China). The plates were incubated at 37°C for 12∼18 h, then the cells were washed from the filter membrane to 1 mL of LB broth. After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China). The colonies after overnight culture at 37°C were transconjugants (Ec15103-Ec600), as verified by PCR.



    Similar Products

    lb nutrient agar medium containing ampicillin  (Beijing Solarbio Science)
    90
    Beijing Solarbio Science lb nutrient agar medium containing ampicillin
    Mics of antibiotics and compounds.
    Lb Nutrient Agar Medium Containing Ampicillin, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lb+nutrient+agar+medium+containing+ampicillin/lb+nutrient+agar+medium+containing+ampicillin/pmc10464524-64-15-16
    Average 90 stars, based on 1 article reviews
    lb nutrient agar medium containing ampicillin - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Mics of antibiotics and compounds.

    Journal: Frontiers in Microbiology

    Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China

    doi: 10.3389/fmicb.2023.1158659

    Figure Lengend Snippet: Mics of antibiotics and compounds.

    Article Snippet: After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China).

    Techniques:

    Relative changes in resistance gene mRNA levels in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 under the influence of antibiotics. (A,B) Relative changes in bla CTX–M–14 and bla CTX–M–64 mRNA levels in strain Ec15103 under different concentrations of cefotaxime (CTX); bla CTX–M–14 is present on the Ec15103 plasmid pEc15103B and bla CTX–M–64 on the Ec15103 chromosome. (C) Relative changes in bla CTX–M–14 mRNA levels in strain Ec15103-Ec600 at different concentrations of CTX. bla CTX–M–14 is located on the Ec15103-Ec600 conjugate plasmid pEc15103B. (D) Relative changes in bla TEM–1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of ampicillin (AMP). bla TEM–1 is located on the Ec15103-EPI300-1 transconjugant plasmid pEc15103A. For at least two independent experiments, each with at least three biological replicates, the data shown represent the mean standard deviation using one-way ANOVA.

    Journal: Frontiers in Microbiology

    Article Title: Characterizations of bla CTX–M–14 and bla CTX–M–64 in a clinical isolate of Escherichia coli from China

    doi: 10.3389/fmicb.2023.1158659

    Figure Lengend Snippet: Relative changes in resistance gene mRNA levels in strains Ec15103, Ec15103-Ec600, and Ec15103-EPI300-1 under the influence of antibiotics. (A,B) Relative changes in bla CTX–M–14 and bla CTX–M–64 mRNA levels in strain Ec15103 under different concentrations of cefotaxime (CTX); bla CTX–M–14 is present on the Ec15103 plasmid pEc15103B and bla CTX–M–64 on the Ec15103 chromosome. (C) Relative changes in bla CTX–M–14 mRNA levels in strain Ec15103-Ec600 at different concentrations of CTX. bla CTX–M–14 is located on the Ec15103-Ec600 conjugate plasmid pEc15103B. (D) Relative changes in bla TEM–1 mRNA levels in strain Ec15103-EPI300-1 at different concentrations of ampicillin (AMP). bla TEM–1 is located on the Ec15103-EPI300-1 transconjugant plasmid pEc15103A. For at least two independent experiments, each with at least three biological replicates, the data shown represent the mean standard deviation using one-way ANOVA.

    Article Snippet: After 1,000-fold dilution, 100 μL was spread on LB nutrient agar medium containing 100 μg/mL ampicillin (Solarbio Science & Technology, Beijing, China) and 750 μg/mL rifampicin (Solarbio Science & Technology, Beijing, China).

    Techniques: Plasmid Preparation, Standard Deviation